PMSG profiles in superovulated and anti-PMSG antiserum treated mice and heifers with enzymeimmunoassay.

نویسندگان

  • S Katagiri
  • Y Takahashi
  • M Hishinuma
  • H Kanagawa
  • O Dochi
  • H Takakura
چکیده

A sandwich enzymeimmunoassay (EIA) for pregnant mare serum gonadotropin (PMSG) using a microtiter plate was developed. Sensitivity of the assay to PMSG was 15.6 mIU/ml (0.2 ng/well). The PMSG levels in serum were measured with the EIA in superovulated and anti-PMSG rabbit antiserum treated mice and heifers. In mice, the PMSG blood level was measurable in the serum 4-6 days after intraperitoneal injection of 5-30 IU of PMSG. The administration of anti-PMSG antiserum at the same dose level as PMSG caused a rapid decrease in the PMSG blood level, declining to undetectable levels within 17 hours. In heifers, the PMSG level was measurable at 10-11 days after the injection of 2500 or 3000 IU of PMSG. When antiserum was injected 48 hours after the PMSG injection, the clearance rate of PMSG was affected by the route of the administration. The administration of 3000 units of anti-PMSG antiserum intravenously caused a rapid decline and the disappearance of circulating PMSG within 17 hours. When 3000 units of anti-PMSG antiserum was injected intra-muscularly, the PMSG blood level also decreased and became unmeasurable 24 hours after administration; however, it was still detectable for up to 17 hours. These results indicate that the administration of anti-PMSG antiserum at the proper timing and dosage could lead to successful superovulation through the improvement of hormonal conditions.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Fertilizability of Superovulated Eggs by Estrous Stage-independent PMSG/hCG Treatment in Adult Wistar-Imamichi Rats

We investigated the fertilization and developmental ability of superovulated eggs obtained from adult Wistar-Imamichi (WI) rats, by using pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) treatment. Female WI rats, 11-13 weeks of age, were divided into four groups by estrous stage (metestrus [ME], diestrus [DE], proestrus [PE], or estrus [E]). PMSG (150 IU/kg) and h...

متن کامل

Ovulation, fertility and prenatal mortality in heifers treated with PMSG or porcine FSH.

Ovulation rate, fertilization rate and prenatal mortality were studied in yearling beef heifers treated with PMSG or FSH and in non-treated controls. Oestrus was synchronized in 50% of the animals in each treatment with an oral progestagen. The gonadotrophin injections were given during the normal oestrous cycle or during the progestagen-administration period. All heifers were inseminated 6 to ...

متن کامل

Endocrine and reproductive response of beef cows to PMSG.

Thir ty-one 3-year-old Angus cows and 23 2-year-old Angus heifers were randomly allotted to two t rea tment groups to examine the influence o f PMSG on endocr ine and reproductive responses. Sixteen cows and 11 heifers received a single subcutaneous inject ion of 2,000 IU PMSG on day 17 o f the estrous cycle (Trt 17); 15 cows and 12 heifers received 1,500 IU PMSG on day 5 and 2,000 IU on day 17...

متن کامل

Biochemical status of ovaries after induction of superovulation on different days of estrus cycle in mice.

To investigate the role of ovarian status and to find out a suitable hormonal dose for induction of superovulation and its effect on biochemical status of the ovaries, the mice were injected with PMSG in doses of 5, 7.5, and 10 IU on different days of the estrous cycle i.e. proestrus, estrus, metestrus and diestrus followed by hCG injection 48 hr later. All these treatments increased the mean o...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Japanese journal of veterinary research

دوره 39 1  شماره 

صفحات  -

تاریخ انتشار 1991